Separation of Replicative Intermediate from Single-stranded Ribonucleic Acid by Sedimentation at Low Ionic Strength

AUTOR(ES)

Fenwick, M. L.

RESUMO

By sedimentation in 2.5 × 10−4m sodium ethylenediaminetetraacetate at 25 C, replicative intermediate could be separated from single-stranded ribonucleic acid, both viral and ribosomal. All of the label in the replicative intermediate after a brief pulse of titrated uridine was resistant to ribonuclease.

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