Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene.

AUTOR(ES)

Tao, T

RESUMO

An open reading frame partially overlaps pvuIIR, and genetic evidence implies that this open reading frame, named pvuIIC, specifies a positive regulator of pvuIIR (T. Tao, J. C. Bourne, and R. M. Blumenthal, J. Bacteriol. 173:1367-1375, 1991). Inducible constructs of pvuIIC produced a protein of the expected size. The site of C.PvuII action appears to lie within pvuIIC itself; thus, pvuIIC may be a self-contained regulatory cassette.

Documentos Relacionados

• The inhibition of restriction endonuclease PvuII cleavage activity by methylation outside its recognition sequence.
• PvuII RFLP inside the human estrogen receptor gene.
• A PvuII RFLP in the human ADH2 gene.
• Alteration of the specificity of PvuII restriction endonuclease.
• PvuII polymorphic site upstream to the human ApoCIII gene.