Sequence and expression of four mutant aspartic acid tRNA genes from the mitochondria of Saccharomyces cerevisiae.
AUTOR(ES)
Najarian, D
RESUMO
Expression of the mitochondrial tRNAAsp gene of Saccharomyces cerevisiae has been examined in five syn- mutants known to affect tRNAAsp function, and in a rho- mutant which accumulates precursor tRNAs. By comparison of wild-type versus mutant DNA sequence, the lesion in each syn- mutant has been identified as a single base change within the mitochondrial tRNAAsp structural gene. The mutant tRNAAsp genes are transcribed, and the transcripts can be processed to mature 4S-size tRNAAsp. The steady-state level of each mutant tRNAAsp is lower than that of wild-type tRNAAsp. The RNA from two of the syn- mutants contained a second, slow-migrating form of mitochondrial tRNAAsp which is correctly processed at the 5' end. We conclude that the lesions in the syn- mitochondrial tRNAAsp genes block neither transcription of these genes, nor 5'-end processing of the transcripts. The effect of each point mutation must be manifested at the level of 3'-end processing, or at a functional level.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341321Documentos Relacionados
- Isopentenyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae.
- Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevisiae.
- Transcripts of mitochondrial tRNA genes in Saccharomyces cerevisiae.
- Role of nuclear genes in expression of a mitochondrial tRNA gene in Saccharomyces cerevisiae.
- Expression and function of a human initiator tRNA gene in the yeast Saccharomyces cerevisiae.