Sequence and position requirements for uridylate-rich downstream elements of polyadenylation signals.
AUTOR(ES)
Chou, Z F
RESUMO
We have defined the positional and sequence requirements of U-rich downstream elements using a simian virus 40 late polyadenylation signal containing a substituted downstream region. A UUUUU element will significantly increase the efficiency of 3' end processing when placed between 6 and 25 bases downstream from the cleavage site. Positions in this interval closer than 15 bases from the cleavage site, however, were noticeably less efficient. Placement of the UUUUU element between +20 and +25 caused a partial shift in cleavage site usage to a CA motif at +4. Mutational analysis indicated that the sequence requirements at individual positions of the UUUUU element were somewhat flexible. Changing more than one base of the UUUUU sequence, however, severely diminished the ability of the element to mediate efficient 3' end processing. Finally, although hnRNP C proteins specifically interact with U-rich sequences, this protein--RNA interaction is not required for efficient in vitro polyadenylation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=308205Documentos Relacionados
- Occurrence of Uridylate-Rich Oligonucleotide Regions in Heterogeneous Nuclear RNA of HeLa Cells
- Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
- DNA sequences downstream of the adenovirus type 2 fiber polyadenylation site contain transcription termination signals.
- Downstream sequence elements with different affinities for the hnRNP H/H′ protein influence the processing efficiency of mammalian polyadenylation signals
- Polyadenylation and transcription termination in gene constructs containing multiple tandem polyadenylation signals.