Sequence Arrangement in Satellite DNA from the Muskmelon 1
AUTOR(ES)
Bendich, Arnold J.
RESUMO
Two fractions of a satellite DNA from the muskmelon (Cucumis melo L.) isolated as a unimodal peak from CsCl gradients, differ in melting properties and complexity as estimated by reassociation kinetics. At 49.8 C, all of the low melting fraction was denatured and all of the high melting fraction was native. There were almost no partially denatured molecules detected in the electron microscope at this temperature. This observation provides direct evidence that the two fractions are not closely linked. We conclude that satellite I, the high tm, low complexity fraction, exists as a 600-nucleotide sequence in blocks of at least 67 tandem repeats. Since the complexity of the low melting fraction, satellite II, is greater than the size of the molecules in our assay, we can only say that the minimum size of each unit of satellite II is 2.5 × 107 daltons. It is unlikely that any spacer sequences are interspersed with either satellite.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=542457Documentos Relacionados
- Novel Properties of Satellite DNA from Muskmelon
- Satellite 1 DNA sequence from genomic DNA of the giant panda Ailuropoda melanoleuca.
- Sequence arrangement of a highly methylated satellite DNA of a plant, Scilla: A tandemly repeated inverted repeat
- DNA sequence adjacent to and specific for the 1.672 g/cm3 satellite DNA in the Drosophila genome.
- DNA sequence of a Drosophila satellite associated sequence.