Sequence similarity between the viral cp gene and the transgene in transgenic papayas.
AUTOR(ES)
SOUZA JÚNIOR, M.T.
FONTE
Pesquisa Agropecuária Brasileira
DATA DE PUBLICAÇÃO
2011
RESUMO
The Papaya ringspot virus (PRSV) coat protein transgene present in 'Rainbow' and 'SunUp' papayas disclose high sequence similarity (>89%) to the cp gene from PRSV BR and TH. Despite this, both isolates are able to break down the resistance in 'Rainbow', while only the latter is able to do so in 'SunUp'. The objective of this work was to evaluate the degree of sequence similarity between the cp gene in the challenge isolate and the cp transgene in transgenic papayas resistant to PRSV. The production of a hybrid virus containing the genome backbone of PRSV HA up to the Apa I site in the NIb gene, and downstream from there, the sequence of PRSV TH was undertaken. This hybrid virus, PRSV HA/TH, was obtained and used to challenge 'Rainbow', 'SunUp', and an R2 population derived from line 63-1, all resistant to PRSV HA. PRSV HA/TH broke down the resistance in both papaya varieties and in the 63-1 population, demonstrating that sequence similarity is a major factor in the mechanism of resistance used by transgenic papayas expressing the cp gene. A comparative analysis of the cp gene present in line 55-1 and 63-1-derived transgenic plants and in PRSV HA, BR, and TH was also performed.
ASSUNTO(S)
carica papaya potyvirus resistance breeding virus carica papaya potyvirus resistance breeding
ACESSO AO ARTIGO
http://www.alice.cnptia.embrapa.br/handle/doc/115451Documentos Relacionados
- Multiplex gene regulation: a two-tiered approach to transgene regulation in transgenic mice.
- Transgenic Mouse with the Herpes Simplex Virus Type 1 Latency-Associated Gene: Expression and Function of the Transgene
- DNA Sequence Similarity between California Isolates of Cryptosporidium parvum
- Sequence similarity between stereocilin and otoancorin points to a unified mechanism for mechanotransduction in the mammalian inner ear
- Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein.