Sequence-specific DNA purification by triplex affinity capture.
AUTOR(ES)
Ito, T
RESUMO
A DNA isolation procedure was developed by using triple-helix formation and magnetic separation. In this procedure, target DNA is captured by a biotinylated oligonucleotide via intermolecular triplex formation, bound to streptavidin-coated magnetic beads, and recovered in double-stranded form by elution with a mild alkaline buffer that destabilizes the triple helix. The effectiveness of the procedure was demonstrated by a model experiment with an artificially reconstructed library and, also, by the isolation of (dT-dC)n.(dG-dA)n dinucleotide repeats from a human genomic library. This procedure provides a prototype for other triplex-mediated DNA isolation technologies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=48265Documentos Relacionados
- Affinity purification of sequence-specific DNA binding proteins.
- A high-capacity column for affinity purification of sequence-specific DNA-binding proteins.
- Sequence-specific DNA double-strand breaks induced by triplex forming 125I labeled oligonucleotides.
- Sequence-specific DNA-binding dominated by dehydration.
- Sequence-specific DNA binding by Myc proteins.
