Sequence studies of nonradioactive Mycoplasma tRNAPhe with the aid of polynucleotide phosphorylase and polynucleotide kinase

AUTOR(ES)

Szeto, Kwok S.

RESUMO

The known methods of enzymatic phosphorylation with [32P]phosphate of the 3′- or 5′-hydroxyl group of an oligonucleotide have been applied to oligonucleotides derived from Mycoplasma tRNAPhe. The fingerprints obtained by both methods are very similar to each other and to that of uniformly labelled tRNA. The sequence of some oligonucleotides was determined by partial digestion of the 3′-phosphorylated fragment with spleen phosphodiesterase and of the corresponding 5′-phosphorylated fragment with venom phosphodiesterase.

Documentos Relacionados

• Fingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase
• Luminescence and Binding Studies on tRNAPhe
• Conformation of Charged and Uncharged tRNAPhe
• The conformation of the tRNAPhe anticodon loop monitored by fluorescence.
• A novel conformational change of the anticodon region of tRNAPhe (yeast).