Sequences involved in accurate and efficient transcription of human c-myc genes microinjected into frog oocytes.
AUTOR(ES)
Nishikura, K
RESUMO
By microinjecting a series of deletion mutant constructs into Xenopus laevis oocytes, transcriptional control regions, two promoters, of the human c-myc gene were defined. In the case of the first promoter, sequences between -60 and -37 relative to the transcription start site contained an element essential for promoter activity. In the case of the second promoter, sequences between -66 and -56 relative to the initiation site appeared to be involved in accurate and efficient transcription. In both cases, the region identified as the essential promoter element contained GGGCGG or GGCGGG,GC box-like sequences, suggesting that c-myc gene promoter activity may be controlled by transcription factor Sp1 binding in the microinjected oocytes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367176Documentos Relacionados
- Accurate and efficient transcription of human c-myc genes injected into Xenopus laevis oocytes.
- Transcription elongation in the human c-myc gene is governed by overall transcription initiation levels in Xenopus oocytes.
- c-Myc Target Genes Involved in Cell Growth, Apoptosis, and Metabolism
- Amplification units containing human N-myc and c-myc genes.
- Regulation of translocated c-myc genes transfected into plasmacytoma cells.