Sequences spanning the EcoRI substrate site.
AUTOR(ES)
Garfin, D E
RESUMO
Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair sequence overlapping the EcoRI site of Col El plasmid DNA. Three other DNAs were similarly tested, although extended sequences were not determined in these cases. The EcoRI site was shown to be symmetric double-stranded equivalent of -N-G-A-A-T-T-C-N-.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=343552Documentos Relacionados
- Substrate dependence of the mechanism of EcoRI endonuclease.
- Specificity of substrate recognition by the EcoRI restriction endonuclease.
- Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes.
- Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites.
- Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.