Serological and Immunogenic Activity of Soluble Mouse Transplantation Antigens Controlled by the H-2 Locus

AUTOR(ES)

Strober, Samuel

RESUMO

The solubilization and partial purification of mouse transplantation antigens were monitored by (1) an in vitro assay for alloantigenic specificities and (2) an in vivo assay for transplantation antigens controlled by the H-2 histocompatibility locus. Antigens from A/J mice were solubilized by papain and fractionated on a Sephadex G-150 column. The eluate showed a 280 nm absorbance peak (F1) in the excluded volume and two peaks (F2 and F3) in the included volume. H-2 specificities 1, 3, 4, 5, 11, 23, and 28 were confined to a single peak in the F2 fraction.

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