Significance of constitutive integrase synthesis.
AUTOR(ES)
Campbell, A
RESUMO
One conceivable function for constitutive integrase formation by lambda prophage is to stabilize the inserted state by catalyzing reinsertion of prophages that are accidentally excised. As this hypothesis implies a dynamic equilibrium betwen inserted and noninserted DNA, the existence of such an equilibrium is explored. By examining the frequency with which prophages appear in an initially unoccupied chromosomal site of a lysogenic bacterium in which the prophage attachment site is duplicated, the off-rate is estimated as less than 10(-2) per generation for wild-type lambda, and less than 4 x 10(-4) for N- mutants of lambda. From the rate of integrase-catalyzed haploidization of certain partial diploid strains, the rate of spontaneous integrase activity is estimated as 3 x 10(-3) per generation. From these values I conclude that constitutive integrase will not appreciably stabilize the inserted state by virtue of its known activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=336024Documentos Relacionados
- Isolation of Saccharomyces cerevisiae mutants constitutive for invertase synthesis.
- Hyper-recombination in Escherichia coli K-12 mutants constitutive for protein X synthesis.
- Isolation and characterization of a mutant ColE1 plasmid that allows constitutive colicin E1 synthesis.
- Regulation of arginine biosynthesis in Saccharomyces cerevisiae: isolation of a cis-dominant, constitutive mutant for ornithine carbamoyltransferase synthesis.
- Synchrony of gene expression and the differentiation of myeloid leukemic cells: reversion from constitutive to inducible protein synthesis.