Simian virus 40 origin DNA-binding domain on large T antigen.
AUTOR(ES)
Paucha, E
RESUMO
Fifty variant forms of simian virus 40 (SV40) large T antigen bearing point, multiple point, deletion, or termination mutations within a region of the protein thought to be involved in DNA binding were tested for their ability to bind to SV40 origin DNA. A number of the mutant large T species including some with point mutations were unable to bind, whereas many were wild type in this activity. The clustering of the mutations that are defective in origin DNA binding both reported here and by others suggests a DNA-binding domain on large T maps between residues 139 and approximately 220, with a particularly sensitive sequence between amino acids 147 and 166. The results indicate that the domain is involved in binding to both site I and site II on SV40 DNA, but it remains unclear whether it is responsible for binding to cellular DNA. Since all the mutants retain the ability to transform Rat-1 cells, we conclude that the ability of large T to bind to SV40 origin DNA is not a prerequisite for its transforming activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252698Documentos Relacionados
- Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.
- Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
- Properties of the DNA-binding domain of the simian virus 40 large T antigen.
- The Origin DNA-Binding and Single-Stranded DNA-Binding Domains of Simian Virus 40 Large T Antigen Are Distinct
- The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.