Simple Method for the Preparation of Streptococcal Nucleases
AUTOR(ES)
Marker, Stephen C.
RESUMO
A simple method for preparation and fractionation of the streptococcal nucleases by polyacrylamide gel electrophoresis is presented. The procedure is carried out with ammonium sulfate-precipitated supernatant fluids from cultures of beta-hemolytic streptococci grown to stationary phase. Electrophoresis on polyacrylamide gel and subsequent elution of the fractionated enzymes allows the preparation of sufficient homogeneous nuclease B for a large number of anti-nuclease B titrations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=380346Documentos Relacionados
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