Simple Procedure for Production by Group C Streptococci of Phage-Associated Lysin Active Against Group A Streptococci
AUTOR(ES)
Cohen, Jay O.
RESUMO
Phage-associated lysin of high potency was prepared by growing the host group C streptococcal strain 26RP66 in a semisynthetic medium. The lysin was stabilized by adding dithiothreitol and neutralized ethylenediaminetetraacetic acid (EDTA) to facilitate further concentration and partial purification. The lysin remained active when stored at -65 C for 1 year. Lysin was active against all strains of group A streptococci tested and was more active against living cells than heat-killed cells. The procedure outlined is practicable for most bacteriological research laboratories and does not require column purification or other complex biochemical procedures. It should be useful to any laboratory which requires small amounts of lysin to produce L-forms and protoplasts or to release streptococcal antigens.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=186940Documentos Relacionados
- Induction of Lysogenic Bacteriophage and Phage-Associated Toxin from Group A Streptococci during Coculture with Human Pharyngeal Cells
- Phage-associated differences in staphylococcal enterotoxin A gene (sea) expression correlate with sea allele class.
- ENZYMATIC PROPERTIES OF A PHAGE-INDUCED LYSIN AFFECTING GROUP A STREPTOCOCCI
- Identification of a lysin associated with a bacteriophage (A25) virulent for group A streptococci.
- Dissemination of the Phage-Associated Novel Superantigen Gene speL in Recent Invasive and Noninvasive Streptococcus pyogenes M3/T3 Isolates in Japan