Simplification of Lipase Design in the Enzymatic Kinetic Resolution of Amines by Saturation Transfer Difference NMR
AUTOR(ES)
Silva, Marcio S., Pietrobom, Daniel
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2016-10
RESUMO
In this work, we demonstrate a nuclear magnetic resonance (NMR) method for racemic amide and lipase interaction as a first-pass design method in the enzymatic kinetic resolution of amines. As a novel adaptation of commonly used protein-ligand screening NMR methodologies, this approach relies upon a lipase-amide interaction wherein the time-consuming is reduced drastically and new insights are produced during the development of biocatalysis reactions.
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