Simultaneous Use of Contrasting Fluorochromes to Separate Measles and Canine Distemper Viruses in a Common System

AUTOR(ES)

Gourlay, James A.

RESUMO

Measles and canine distemper viruses were grown together in a Vero monkey kidney cell line. Each virus could be identified and individually titrated by using the color contrast produced by the reddish tetra-methyl rhodamine isothiocyanate-tagged antimeasles conjugate and the green fluorescein isothiocyanate-tagged antidistemper conjugate. Both blue light and green light were used for the excitation of the fluorochromes. Incident light was transmitted to the specimen by a vertical illuminator of the Ploem type.

Documentos Relacionados

• A study of immunoglobulin M antibody to measles, canine distemper, and rinderpest viruses in sera of patients with subacute sclerosing panencephalitis.
• Antigenic relationships between measles and canine distemper viruses: comparison of immune response in animals and humans to individual virus-specific polypeptides.
• IMMUNOLOGICAL RELATIONSHIPS OF MEASLES, DISTEMPER, AND RINDERPEST VIRUSES*
• SUBCLINICAL INFECTION OF DOGS BY CANINE-ADAPTED MEASLES VIRUS EVIDENCED BY THEIR SUBSEQUENT IMMUNITY TO CANINE DISTEMPER VIRUS
• Establishment of a Rescue System for Canine Distemper Virus