Simultaneous visualization of aortic and [3H]5-hydroxytryptamine-accumulating cell bodies in the nodose ganglion of the cat

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RESUMO

1. Single- and double-tracer experiments were performed in cats to investigate the relationship between the aortic cells and the cell bodies accumulating 5-hydroxytryptamine (5-HT) in the nodose ganglion. In one series of experiments, horseradish peroxidase (HRP) was applied to the central end of the aortic nerve, anterogradely transported and accumulated in ganglionar perikarya. The distribution of HRP-positive neurones was reconstructed in serial sections through the nodose ganglion. In a second series of experiments, the distribution of [3H]5-HT-accumulating cell bodies was assessed following incubation of the nodose ganglion in [3H]5-HT. The third series of experiments combined the treatments of the preceding ones: anterograde transport of HRP in the aortic nerve followed by incubation of the nodose ganglion in [3H]5-HT.

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