Single-base mutations at position 2661 of Escherichia coli 23S rRNA increase efficiency of translational proofreading.
AUTOR(ES)
Melançon, P
RESUMO
Two single-base substitutions were constructed in the 2660 loop of Escherichia coli 23S rRNA (G2661-->C or U) and were introduced into the rrnB operon cloned in plasmid pKK3535. Ribosomes were isolated from bacteria transformed with the mutated plasmids and assayed in vitro in a poly(U)-directed system for their response to the misreading effect of streptomycin, neomycin, and gentamicin, three aminoglycoside antibiotics known to impair the proofreading control of translational accuracy. Both mutations decreased the stimulation of misreading by these drugs, but neither interfered with their binding to the ribosome. The response of the mutant ribosomes to these drugs suggests that the 2660 loop, which belongs to the elongation factor Tu binding site, is involved in the proofreading step of the accuracy control. In vivo, both mutations reduced read-through of nonsense codons and frameshifting, which can also be related to the increased efficiency in proofreading control which they confer to ribosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=207523Documentos Relacionados
- Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity.
- Mutations in the peptidyl transferase region of E. coli 23S rRNA affecting translational accuracy.
- Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coli.
- A single base mutation at position 2661 in E. coli 23S ribosomal RNA affects the binding of ternary complex to the ribosome.
- Ordered processing of Escherichia coli 23S rRNA in vitro.