Single-Nucleotide-Polymorphism Mapping of the Pseudomonas aeruginosa Type III Secretion Toxins for Development of a Diagnostic Multiplex PCR System

AUTOR(ES)

Ajayi, Temitayo

FONTE

American Society for Microbiology

RESUMO

We mapped the coding single nucleotide polymorphisms in four toxin genes—exoS, exoT, exoU, and exoY—of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and specificity in this small sample size. This assay appears to be promising for the rapid and accurate genotyping of the presence of these genes in clinical strains of P. aeruginosa.

Documentos Relacionados

• Macrophages and Epithelial Cells Respond Differently to the Pseudomonas aeruginosa Type III Secretion System
• Pseudomonas aeruginosa ExoU, a Toxin Transported by the Type III Secretion System, Kills Saccharomyces cerevisiae
• A secreted regulatory protein couples transcription to the secretory activity of the Pseudomonas aeruginosa type III secretion system
• A Three-Component Regulatory System Regulates Biofilm Maturation and Type III Secretion in Pseudomonas aeruginosa†
• Protein Binding between PcrG-PcrV and PcrH-PopB/PopD Encoded by the pcrGVH-popBD Operon of the Pseudomonas aeruginosa Type III Secretion System