Single radial immune hemolysis test for detection of Escherichia coli thermolabile enterotoxin.
AUTOR(ES)
Serafim, M B
RESUMO
A single radial immune hemolysis test for the detection of thermolabile enterotoxin has been developed for routine purposes. Stationary cultures from enterotoxigenic Escherichia coli in Casamino Acids-yeast extract medium may be used for the detection of this enterotoxin, and under the conditions of the experiment, the single radial immune hemolysis test was as sensitive as the passive immune hemolysis test. The results obtained in the single radial immune hemolysis test agreed entirely with those obtained in the passive immune hemolysis test, and no false-positive reactions were obtained when cholera antitoxin diluted 1:80 was used. The assay is easy to perform, inexpensive, and specially designed for less-equipped laboratories.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273971Documentos Relacionados
- Radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae.
- Single radial immunodiffusion method for screening Staphylococcal isolates for enterotoxin.
- Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.
- Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources.
- Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
