Single-serum diagnosis of rubella by combined use of the hemagglutination inhibition and passive hemagglutination tests.
AUTOR(ES)
Inouye, S
RESUMO
We explored the feasibility of using the passive hemagglutination (PHA) test in combination with the hemagglutination inhibition (HI) test for the single-serum diagnosis of rubella. We found by sedimentation analysis of the serum that early 7S antibody produced within 1 month after the onset of the rash had high HI but much lower PHA titers, whereas the PHA titers of antibody produced later were slightly higher than the HI titers. (19S antibody in the early serum had some PHA activity.) This disparity between the HI and PHA activities of the early 7S antibody could be used for the routine diagnosis of rubella. A comparison of the HI titers of the test serum with the PHA titers of mercaptoethanol-treated serum constitutes a simple method for determining whether the serum sample was taken shortly or remotely after the infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=268653Documentos Relacionados
- Antibody responses in patients with rubella infection determined by passive hemagglutination, hemagglutination inhibition, complement fixation, and solid-phase radioimmunoassay tests.
- Letter: Trypsin-treated human erythrocytes for rubella hemagglutination inhibition tests.
- Immunoglobulin responses to Coxiella burnetii (Q fever): single-serum diagnosis of acute infection, using an immunofluorescence technique.
- Cryopreserved erythrocytes in clinical laboratory hemagglutination and hemagglutination inhibition tests.
- An Improved Diluent for Rubella Hemagglutination and Hemagglutination-Inhibition Tests
