Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae.
AUTOR(ES)
LeClerc, J E
RESUMO
The deoxyribonucleic acid (DNA) of competent wild-type Haemophilus influenzae and rec1 mutant cells contains single-strand regions, as judged by alkaline sucrose sedimentation, benzoylated naphthoylated diethylaminoethyl-cellulose fractionation, and digestion with an enzyme specific for single-strand regions in DNA. In contrast, the DNA of competent rec2 cells does not contain single-strand regions. Since transforming DNA does not associate with recipient DNA in the rec2 mutant as it does in wild type and rec1, it is concluded that the single-strand regions in the DNA of the competent cells are important for an early step in recombination between cell DNA and transforming DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246164Documentos Relacionados
- Repair of Single-Strand Deoxyribonucleic Acid Breaks in Ultraviolet Light-Irradiated Haemophilus influenzae
- Single-stranded regions in transforming deoxyribonucleic acid after uptake by competent Haemophilus influenzae.
- Direct Enzymatic Repair of Deoxyribonucleic Acid Single-Strand Breaks in Dormant Spores
- Repair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid.
- Thymine Starvation and Single-Strand Breaks in Chromosomal Deoxyribonucleic acid of Escherichia coli
