Size and complexity of the nuclear genome of Colletotrichum graminicola.

AUTOR(ES)

Randhir, R J

RESUMO

DNA reassociation was used to estimate GC content, size, and complexity of the nuclear genomes of Colletotrichum from maize and sorghum. Melting-temperature analysis indicated that the GC content of the maize pathotype DNA was 51% and that the GC content of the sorghum pathotype was 52%. DNA reassociation kinetics employing S1 nuclease digestion and an appropriately modified second-order equation indicated that the genome sizes of the maize and sorghum pathotypes were 4.8 x 10(7) bp, and 5.0 x 10(7) bp, respectively. Genomic reconstruction experiments based on Southern blot hybridization between a cloned single-copy gene, PYR1 (orotate phosphoribosyl transferase), and maize-pathotype DNA confirmed the size of the nuclear genome. The single-copy component of the genomes of both pathotypes was estimated at about 90%. For both pathotypes, ca. 7% of the genome represented repetitive DNA, and 2 to 3% was foldback DNA.

Documentos Relacionados

• Race diversity and complexity in populations of the sorghum anthracnose fungus Colletotrichum graminicola.
• Caracterização molecular de microrganismos do ecossistema agricola do cerrado. II. Colletotrichum graminicola.
• Genome Size, Complexity, and Ploidy of the Pathogenic Fungus Histoplasma capsulatum
• Antracnose do sorgo (Colletotrichum graminicola).
• Antracnose do milho (Colletotrichum graminicola).