Slipped-Strand Mispairing Can Function as a Phase Variation Mechanism in Escherichia coli

AUTOR(ES)

Torres-Cruz, Joshua

FONTE

American Society for Microbiology

RESUMO

Slipped-strand mispairing (SSM) has not been identified as a mechanism of phase variation in Escherichia coli. Using a reporter gene, we show that sequences that cause phase variation by SSM in Haemophilus influenzae also lead to phase variation when introduced onto the chromosome of E. coli, and the frequencies of switching are in the biologically relevant range. Thus, the absence of SSM-mediated phase variation in E. coli does not appear to be due to a mechanistic constraint.

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