Small, acid-soluble proteins bound to DNA protect Bacillus subtilis spores from killing by dry heat.

AUTOR(ES)

Setlow, B

RESUMO

Dry Bacillus subtilis spores lacking their two major DNA-binding proteins (small, acid-soluble proteins [SASP] alpha and beta) were much more sensitive to dry heat than were wild-type spores. Survivors of dry heat treatment of both wild-type and mutant spores exhibited a high frequency of mutations, and the DNA from the heated spores had increased numbers of single-strand breaks. These data indicate that SASP alpha and beta provide significant protection to spore DNA against the damaging effects of dry heat. This DNA damage may be in part depurination, and a purified alpha/beta-type SASP gave significant protection against dry heat-induced DNA depurination in vitro.

Documentos Relacionados

• Small, acid-soluble proteins bound to DNA protect Bacillus subtilis spores from being killed by freeze-drying.
• Cloning and nucleotide sequencing of genes for three small, acid-soluble proteins from Bacillus subtilis spores.
• Prevention of DNA damage in spores and in vitro by small, acid-soluble proteins from Bacillus species.
• Heat inactivation of Bacillus subtilis spores lacking small, acid-soluble spore proteins is accompanied by generation of abasic sites in spore DNA.
• Small, Acid-Soluble Spore Proteins of the α/β Type Do Not Protect the DNA in Bacillus subtilis Spores against Base Alkylation