Some characteristics of apurinic sites in single- and double-stranded biologically active phi X174 DNA.
AUTOR(ES)
Lafleur, M V
RESUMO
With biologically active DNA of the bacteriophage phi X174, both single and double-stranded, some physico-chemical and biological parameters of the depurination reaction are studied. It is shown that in single-stranded DNA each apurinic site is lethal, while in double-stranded RFI-DNA only about 5% of these sites are lethal. Furthermore it is concluded that the apurinic sites are formed at different rates in single- and double-stranded DNA and also the conversion into breaks of the apurinic sites is different for both forms of DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327624Documentos Relacionados
- Atomic force microscopy of single- and double-stranded DNA.
- Local Conformations and Competitive Binding Affinities of Single- and Double-stranded Primer-Template DNA at the Polymerization and Editing Active Sites of DNA Polymerases*
- Regions of incompatibility in single-stranded DNA bacteriophages phi X174 and G4.
- Initiation and termination of the bacteriophage phi X174 rolling circle DNA replication in vivo: packaging of plasmid single-stranded DNA into bacteriophage phi X174 coats.
- Extrachromosomal recombination in mammalian cells as studied with single- and double-stranded DNA substrates.
