Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea
AUTOR(ES)
Fitzgerald, Nancy B.
FONTE
American Society for Microbiology
RESUMO
We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=106195Documentos Relacionados
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