Sorbitol Metabolism in Aerobacter aerogenes

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RESUMO

Sorbitol (d-glucitol) metabolism in Aerobacter aerogenes PRL-R3 is shown to proceed via the pathway: sorbitol → sorbitol 6-phosphate → d-fructose 6-phosphate. Sorbitol phosphorylation is mediated by a phosphoenolpyruvate (PEP):sorbitol 6-phosphotransferase system, and sorbitol 6-phosphate oxidation by a pyridine-nucleotide-linked dehydrogenase. Mutants deficient in sorbitol 6-phosphate dehydrogenase or a component (enzyme I) of the phosphotransferase system did not grow on sorbitol, whereas revertants which had regained these enzymatic activities grew normally. Extracts of the enzyme I-deficient mutant failed to catalyze the phosphorylation of sorbitol in the presence of PEP, and adenosine 5′-triphosphate could not replace the PEP requirement for sorbitol phosphorylation in extracts of the wild-type strain.

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