Species-specific monoclonal antibodies in the assignment of the gene for human fibronectin to chromosome 2.

AUTOR(ES)

Zardi, L

RESUMO

Using three different species-specific monoclonal antibodies we have studied, in human-mouse and human-hamster somatic cell hybrids, the correlation between the presence of different human chromosomes and the ability to release human fibronectin into the tissue culture medium. Presence of human fibronectin was determined by an affinity-radioimmunoassay. In addition, tissue culture media of the different hybrids were separated on SDS-polyacrylamide gels, the proteins were blotted onto a nitrocellulose sheet and human fibronectin visualized by an immunoenzymatic technique. Karyology and determination of isoenzyme markers of specific human chromosomes show that the ability to produce human fibronectin segregated with the presence of human chromosome 2.

Documentos Relacionados

• Species-Specific Monoclonal Antibodies for Rapid Identification of Bartonella quintana
• Tissue-specific and species-specific monoclonal antibodies to avian red cell nuclear proteins.
• Monoclonal antibodies to Legionella Mip proteins recognize genus- and species-specific epitopes.
• Species-specific monoclonal antibodies to Rickettsia japonica, a newly identified spotted fever group rickettsia.
• Preparation of species-specific murine monoclonal antibodies against the yeast phase of Paracoccidioides brasiliensis.