Specific DNA binding of the two chicken Deformed family homeodomain proteins, Chox-1.4 and Chox-a.
AUTOR(ES)
Sasaki, H
RESUMO
The cDNA clones encoding two chicken Deformed (Dfd) family homeobox containing genes Chox-1.4 and Chox-a were isolated. Comparison of their amino acid sequences with another chicken Dfd family homeodomain protein and with those of mouse homologues revealed that strong homologies are located in the amino terminal regions and around the homeodomains. Although homologies in other regions were relatively low, some short conserved sequences were also identified. E. coli-made full length proteins were purified and used for the production of specific antibodies and for DNA binding studies. The binding profiles of these proteins to the 5'-leader and 5'-upstream sequences of Chox-1.4 and Chox-a coding regions were analyzed by immunoprecipitation and DNase I footprint assays. These two Chox proteins bound to the same sites in the 5'-flanking sequences of their coding regions with various affinities and their binding affinities to each site were nearly the same. The consensus sequences of the high and low affinity binding sites were TAATGA(C/G) and CTAATTTT, respectively. A clustered binding site was identified in the 5'-upstream of the Chox-a gene, suggesting that this clustered binding site works as a cis-regulatory element for auto- and/or cross-regulation of Chox-a gene expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=330591Documentos Relacionados
- The nucleotide sequence of the cDNA encoding a chicken Deformed family homeobox gene, Chox-Z.
- RFX proteins, a novel family of DNA binding proteins conserved in the eukaryotic kingdom.
- DNA intercalators induce specific release of HMG 14, HMG 17 and other DNA-binding proteins from chicken erythrocyte chromatin.
- DNA binding specificity of two homeodomain proteins in vitro and in Drosophila embryos.
- Phactrs 1–4: A family of protein phosphatase 1 and actin regulatory proteins