Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
AUTOR(ES)
Schultz, M C
RESUMO
A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=50943Documentos Relacionados
- Chromatin assembly in a yeast whole-cell extract
- Transcriptional activation in an improved whole-cell extract from Saccharomyces cerevisiae.
- DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
- Detection of mRNA in Streptomyces Cells by Whole-Cell Hybridization with Digoxigenin-Labeled Probes
- Analysis of genetic complementation by whole-cell microtechniques in fibroblast heterokaryons.
