Specificity of the DNA Product of RNA-Dependent DNA Polymerase in Type C Viruses: II. Quantitative Analysis*
AUTOR(ES)
Okabe, Hiromi
RESUMO
A number of mammalian Type C viruses were analyzed for relatedness by the technique of DNA·RNA hybridization. Viral DNAs were prepared in single-stranded form from complexes with 70S viral RNA formed during endogenous polymerase reactions. Extent of hybridization was assayed with the single-strand nuclease (S-1) from Aspergillus oryzae. Results obtained indicated a high degree of viral specificity, with significant cross-reactions being observed only with viruses obtained from within a species, as in the case of mouse and cat viruses, or in the special case of woolly monkey-gibbon comparisons. Comparisons of RD-114 virus, recently determined to be of feline origin, and conventional feline Type C viruses (FeLV), revealed minimal relatedness, especially when feline virus was grown on human cells, thus indicating the possibility of coexistence of greatly disparate Type C viruses within one species. A rat-specific virus, recovered from tumors induced by murine sarcoma virus, was found to contain genetic material common to both the original mouse virus and viruses indigenous to the rat, even though only rat-specific proteins have been detected during infection by this virus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=427358Documentos Relacionados
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