Specimen Holder to Critical-Point Dry Microorganisms for Scanning Electron Microscopy

AUTOR(ES)

Kurtzman, C. P.

RESUMO

Critical-point drying of microorganisms for scanning electron microscopy can be rapidly and effectively accomplished by use of a newly described specimen holder. Up to eight different samples of spores or vegetative cells are placed between polycarbonate membrane filters in the holder and processed through solvent dehydration and critical-point drying using carbon dioxide without loss or cross contamination of microorganisms. Yeasts, molds, bacteria, and actinomycetes have been successfully processed.

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