Spin-Label Studies of Dynamics of Lipid Alkyl Chains in Biological Membranes: Role of Unsaturated Sites

AUTOR(ES)

Eletr, S.

RESUMO

Cultures of a yeast mutant, deficient in the synthesis of unsaturated fatty acids, were supplemented with either stearolic acid or with any one of three octadecanoic acids having a cis double bond 6, 9, or 11 carbons away from the acyl group. The resulting cells, with lipid alkyl chains well defined with respect to the position and nature of unsaturated sites, were then studied with spin-labeled stearic acids having a N-oxyloxazolidine ring located at 4, 6, 9, or 12 carbons away from the acyl group, and added in vitro to the cellular preparations.

Documentos Relacionados

• Spin-label studies of the excitable membranes of nerve and muscle.
• Spin-label Studies of Membranes in Rye Protoplasts during Extracellular Freezing 12
• Spin-label electron spin resonance studies on the interactions of lysine peptides with phospholipid membranes.
• High-frequency, spin-label EPR of nonaxial lipid ordering and motion in cholesterol-containing membranes
• Spin-Label Electron Spin Resonance Study of the Lipid-Containing Membrane of Influenza Virus