Spinach thioredoxin m inhibits DNA synthesis in fertilized Xenopus eggs.
AUTOR(ES)
Hartman, H
RESUMO
A role for thioredoxin in metazoan DNA synthesis has been assessed by injecting rapidly dividing Xenopus eggs with purified heterologous thioredoxins, which might act as inhibitors if they were to replace resident thioredoxins in some but not all reaction steps. Of 10 tested proteins, spinach chloroplast thioredoxin m is the most potent inhibitor. Eggs cleave and produce cells lacking nuclei. DNA synthesis is severely reduced. Development arrests before gastrulation. In egg extracts, thioredoxin m inhibits incorporation of radioactive dCTP into DNA of sperm nuclei and M13 phage. Inhibition exceeds 90% when thioredoxin m and M13 DNA are preincubated together. The data support the interpretation that thioredoxins normally participate in initiation of metazoan DNA synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=46068Documentos Relacionados
- Periodic DNA synthesis in cell-free extracts of Xenopus eggs.
- Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs.
- Mismatch repair involving localized DNA synthesis in extracts of Xenopus eggs.
- Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.
- Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.