Splicing of a circular yeast pre-mRNA in vitro.
AUTOR(ES)
Schindewolf, C A
RESUMO
We have tested the fate of a circularized synthetic pre-mRNA transcript in a whole cell splicing extract of Saccharomyces cerevisiae. Our results demonstrate that this circular precursor RNA is able to induce spliceosome formation in vitro and that the products of the following splicing reaction are the lariat-shaped intron, and a mature circular mRNA. Thus, it would appear that free 5' and/or 3' ends are not obligatory for a splicing reaction to occur, although we find its efficiency to be strongly influenced by the presence or lack of free ends. To our knowledge, this is the first demonstration that a circular pre-mRNA molecule is recognized as a suitable substrate by an eukaryotic mRNA splicing apparatus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=306821Documentos Relacionados
- Antisense RNA inhibits splicing of pre-mRNA in vitro.
- Ribonucleoprotein complex formation during pre-mRNA splicing in vitro.
- Thiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicing in vitro.
- Alternative splicing of SV40 early pre-mRNA in vitro.
- A yeast tRNA precursor containing a pre-mRNA intron is spliced via the pre-mRNA splicing mechanism.