Spontaneous Unstable UNC-22 IV Mutations in C. ELEGANS Var. Bergerac

AUTOR(ES)

Moerman, D. G.

RESUMO

This paper describes a mutator system in the nematode Caenorhabditis elegans var. Bergerac for the gene unc-22. Of nine C. elegans and two C. briggsae strains tested only the Bergerac BO strain yielded mutant animals at a high frequency and the unc-22 IV gene is a preferred mutational target. The forward spontaneous mutation frequency at the unc-22 locus in Bergerac BO is about 1 x 10-4 , and most of these spontaneous unc-22 mutations revert at frequencies between 2 x 10-3 and 2 x 10 -4. Both the forward mutation frequency and the reversion frequency are sensitive to genetic background. Spontaneous unc-22 mutations derived in a Bergerac background and placed in a primarily Bristol background revert at frequencies of <10-6. When reintroduced into a Bergerac/Bristol hybrid background the mutations once again become unstable.

Documentos Relacionados

• Essential Genes and Deficiencies in the UNC-22 IV Region of CAENORHABDITIS ELEGANS
• Genetic Organization in CAENORHABDITIS ELEGANS: Fine-Structure Analysis of the unc-22 Gene
• The Unc-22(iv) Region of Caenorhabditis Elegans: Genetic Analysis of Lethal Mutations
• Molecular cloning of the muscle gene unc-22 in Caenorhabditis elegans by Tc1 transposon tagging.
• C. ELEGANS unc-105 Mutations Affect Muscle and Are Suppressed by Other Mutations That Affect Muscle