Spore Pool Glutamic Acid as a Metabolite in Germination
AUTOR(ES)
Foerster, Harold F.
RESUMO
Spore glutamic acid pools were examined in dormant and germinating spores using colorimetric and 14C analytical procedures. Germination of spores of Bacillus megaterium (parent strain), initiated by d-glucose, was accompanied by a rapid drop in the level of spore pool glutamate, from 12.0 μg/mg of dry spores to 7.7 μg/mg of dry spores after 30 sec of germination. Similar decreases in extractable spore pool glutamate were observed with l-alanine-initiated germination of B. licheniformis spores. On the other hand, glutamate pools of mutant spores of B. megaterium, with a requirement of γ-aminobutyric acid for spore germination, remained unchanged for 9 min of germination, at which time more than 50% of the spore population had germinated. Evidence for conversion of spore pool glutamate to γ-aminobutyric acid during germination of spores of B. megaterium (parent strain) was obtained.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=251301Documentos Relacionados
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