Stage- and tissue-specific expression of two homeo box genes in sea urchin embryos and adults.
AUTOR(ES)
Dolecki, G J
RESUMO
We report the isolation of two different homeo box genes, HB3 and HB4, from the Hawaiian sea urchin Tripneustes gratilla. DNA sequencing revealed a definitive Antennapedia (Antp) class homeo box in each gene. Southern transfer hybridizations showed the genes to be single-copy. A 5.7-kb transcript of the HB3 gene was found in ovary, testis, small intestine and gastrula poly(A)+ RNA. The HB4 gene produces three transcripts. A 3.7-kb and a 4.4-kb transcript are expressed during embryogenesis. A 3.5-kb transcript appears in each of the adult tissues studied. The HB4 gene appears to be the sea urchin cognate of the Drosophila infrabdominal-7 (iab-7) gene, the mouse Hox 1.7 and Hox 3.2 genes and the Xenopus X1Hbox 6 gene. An examination of Antp class homeo box genes in deuterostomes indicates that a chromosomal duplication has taken place in the evolutionary line leading to the vertebrates after the divergence of the echinoderms. Thus, the sea urchin represents the primitive condition.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=339072Documentos Relacionados
- Molecular cloning of five individual stage- and tissue-specific mRNA sequences from sea urchin pluteus embryos.
- Stage- and tissue-specific expression of the neu oncogene in rat development.
- Stage- and Tissue-Specific Expression of Ethylene Receptor Homolog Genes during Fruit Development in Muskmelon1
- Metallothionein genes MTa and MTb expressed under distinct quantitative and tissue-specific regulation in sea urchin embryos.
- Isolation of two tissue-specific Drosophila paired box genes, Pox meso and Pox neuro.
