Standardized Viral Hemagglutination and Hemagglutination-Inhibition Tests. I. Standardization of Erythrocyte Suspensions
AUTOR(ES)
Hierholzer, John C.
RESUMO
A spectrophotometric procedure for standardizing red blood cell suspensions for use in viral hemagglutination tests is described. The procedure is based on highly reproducible cyanmethemoglobin absorbance readings at 540 nm on any suitable spectrophotometer. Target values for milligrams of cyanmethemoglobin per 100 ml are given for the red cell suspensions of all mammalian and avian species employed in viral hemagglutination tests. By use of these values and a cyanmethemoglobin standard curve for a particular photometer, approximate 4% erythrocyte suspensions can be diluted to any lesser concentration. Coefficients of variation for the various diluted suspensions range from 4 to 7%.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=378094Documentos Relacionados
- Standardized Viral Hemagglutination and Hemagglutination-Inhibition Tests. II. Description and Statistical Evaluation
- Application of freeze-dried monkey erythrocytes to measles viral hemagglutination and hemagglutination-inhibition tests.
- Application of freeze-dried, one-day-old chick erythrocytes to viral hemagglutination and hemagglutination-inhibition tests.
- An Improved Diluent for Rubella Hemagglutination and Hemagglutination-Inhibition Tests
- Analysis of the Diluents Used for Rubella Virus Hemagglutination and Hemagglutination-Inhibition Tests