Stem cell factor stimulates neurogenesis in vitro and in vivo

AUTOR(ES)

Jin, Kunlin

FONTE

American Society for Clinical Investigation

RESUMO

Cerebral ischemia stimulates neurogenesis in proliferative zones of the rodent forebrain. To identify the signaling factors involved, cerebral cortical cultures prepared from embryonic mouse brains were deprived of oxygen. Hypoxia increased bromodeoxyuridine (BrdU) incorporation into cells that expressed proliferation markers and immature neuronal markers and that lacked evidence of DNA damage or caspase-3 activation. Hypoxia-conditioned medium and stem cell factor (SCF), which was present in hypoxia-conditioned medium at increased levels, also stimulated BrdU incorporation into normoxic cultures. The SCF receptor, c-kit, was expressed in neuronal cultures and in neuroproliferative zones of the adult rat brain, and in vivo administration of SCF increased BrdU labeling of immature neurons in these regions. Cerebral hypoxia and ischemia may stimulate neurogenesis through trophic factors, including SCF.

Documentos Relacionados

• Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo
• Reversible expansion of primate mast cell populations in vivo by stem cell factor.
• Thrombin stimulates tumor-platelet adhesion in vitro and metastasis in vivo.
• Glucocorticoids decrease tissue mast cell number by reducing the production of the c-kit ligand, stem cell factor, by resident cells: in vitro and in vivo evidence in murine systems.
• Crystal structure of human stem cell factor: Implication for stem cell factor receptor dimerization and activation