Stepwise Generation of the Natural Oxidant in a Reconstituted Chloroplast System 1

AUTOR(ES)
RESUMO

Isolated chloroplasts which have lost their envelopes and, in consequence, the soluble components which constitute the stroma, will nevertheless evolve O2 when supplied with an artificial oxidant (the Hill reaction). They will also evolve O2 with NADP as the Hill oxidant if supplemented with ferredoxin. With catalytic NADP, continuing O2 evolution can be maintained by the inclusion of a suitable reaction or reaction sequence which reoxidizes NADPH.

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