Stimulation of glutamate receptor protein synthesis and membrane insertion within isolated neuronal dendrites
AUTOR(ES)
Kacharmina, Janet Estee
FONTE
The National Academy of Sciences
RESUMO
The selective subcellular localization of mRNAs to dendrites and the recent demonstration of local protein synthesis have highlighted the potential role of postsynaptic sites in modulation of cell–cell communication. We show that epitope-tagged subunit 2 of the ionotopic glutamate receptor, GluR2, mRNA transfected into isolated hippocampal neuronal dendrites is translated in response to pharmacologic stimulation. Further, confocal imaging of N-terminally labeled GluR2 reveals that the newly synthesized GluR2 protein can integrate into the dendritic membrane with the N terminus externally localized. These data demonstrate that integral membrane proteins can be synthesized in dendrites and can locally integrate into the cell membrane.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=17237Documentos Relacionados
- Serine racemase: Activation by glutamate neurotransmission via glutamate receptor interacting protein and mediation of neuronal migration
- Glutamate translocation of the neuronal glutamate transporter EAAC1 occurs within milliseconds
- Pentameric subunit stoichiometry of a neuronal glutamate receptor.
- In vitro synthesis, glycosylation, and membrane insertion of the four subunits of Torpedo acetylcholine receptor.
- Membrane assembly in vitro: Synthesis, glycosylatio, and asymmetric insertion of a transmembrane protein
