Strain-Dependent Temperature-Sensitive Phase in Crown Gall Tumorigenesis 1

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RESUMO

The effect of high temperature treatments on the early stages of crown gall tumorigenesis in sunflowers was investigated. Treatments of 32 C initiated at various times during the first ten days after infection had a similar effect on tumors induced by Agrobacterium tumefaciens strains B6 and C58. Tumor growth was sensitive to 32 C until 60 to 72 hours after infection and was stimulated by 32 C after that time. Therefore, the “inception phase” for both C58 and B6-induced tumors ends between 60 to 72 hours after infection. In contrast, B6 and C58 tumors varied in their response to 37 C treatments during the first 168 hours after infection. Both C58 and B6 tumors were sensitive to 37 C during the first 72 hours; however, B6 tumors became resistant to 37 C after 96 hours, whereas C58-induced tumors remained sensitive until 144 to 168 hours after infection.

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