Streptomyces viridochromogenes spore germination initiated by calcium ions.
AUTOR(ES)
Eaton, D
RESUMO
Initiation of germination of heat-activated Streptomyces viridochromogenes spore occurs in media containing only calcium ions and organic buffer. The calcium-induced initiation of germination was accompanied by a decrease in absorbance of the spore suspension, an increased rate of endogenous metabolism, the loss of spore carbon, and the loss of heat resistance. Calcium amounts to 0.28% of the dry weight of freshly harvested spores. The amount of calcium remained the same after incubation of spores in water after heat activation. The spore content of calcium doubled after incubation in 0.5 mM CaCl2 for 5 min at 4 degrees C and during calcium-induced germination. Nearly all of the calcim appears to be bound to sites external to the spore membrane, since the chelating agents (ethylenedinitrilo) tetraacetic acid and arsenazo III removed virtually all of the calcium ions. The calcium ions must be present during the entire initiation of germination period. Germination ceases after an (ethylenedinitrilo) tetraacetic acid wash and begins again immediately after addition of calcium ions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=294250Documentos Relacionados
- Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores.
- Modulation of L-type calcium channels by sodium ions.
- Exposure of actin thiols by the removal of tightly held calcium ions.
- High calcium content in Streptomyces spores and its release as an early event during spore germination.
- Activation of calcium current in voltage-clamped rat glomerulosa cells by potassium ions.
