Structural and functional similarities between the replication region of the Yersinia virulence plasmid and the RepFIIA replicons.

AUTOR(ES)

Vanooteghem, J C

RESUMO

We sequenced the minimum replication region of the virulence plasmid pYVe439-80 from a serogroup O:9 Yersinia enterocolitica. This sequence is 68% homologous on a 1,873-nucleotide stretch to the sequence of the RepFIIA replicon of the resistance plasmid R100. The sequence contains two open reading frames, repA and repB, encoding proteins of 33,478 and 9,568 daltons, respectively. The amino acid sequences of the two proteins are 77 and 55% identical, respectively, to proteins RepA1 and RepA2 of the R100 replicon. Analysis of minicells transformed with a copy number mutant demonstrated that the replication region of pYVe439-80 directs the synthesis of a 33-kilodalton protein. Disruption of repA, encoding this protein, abolished replication. Two regions of pYVe439-80 are 76 and 70% homologous, respectively, to the copy number control antisense RNA and to the origin of replication region of R100. A mutation introduced in the pYVe439-80 DNA corresponding to the R100 sequence encoding the copy number control antisense RNA resulted in an increase in copy number, indicating a functional homology between the two replicons.

Documentos Relacionados

• Isolation of the replication and partitioning regions of the Salmonella typhimurium virulence plasmid and stabilization of heterologous replicons.
• Expression of functional elements inserted into the 35S promoter region of infectious cauliflower mosaic virus replicons.
• DNA replication in Physarum polycephalum: bidirectional replication of DNA within replicons.
• Functional relationship between parts of the replication region of plasmid ColE1.
• Construction and characterization of poliovirus subgenomic replicons.