Structural insight into the essential PB1–PB2 subunit contact of the influenza virus RNA polymerase
AUTOR(ES)
Sugiyama, Kanako
FONTE
Nature Publishing Group
RESUMO
Influenza virus RNA-dependent RNA polymerase is a multi-functional heterotrimer, which uses a ‘cap-snatching' mechanism to produce viral mRNA. Host cell mRNA is cleaved to yield a cap-bearing oligonucleotide, which can be extended using viral genomic RNA as a template. The cap-binding and endonuclease activities are only activated once viral genomic RNA is bound. This requires signalling from the RNA-binding PB1 subunit to the cap-binding PB2 subunit, and the interface between these two subunits is essential for the polymerase activity. We have defined this interaction surface by protein crystallography and tested the effects of mutating contact residues on the function of the holo-enzyme. This novel interface is surprisingly small, yet, it has a crucial function in regulating the 250 kDa polymerase complex and is completely conserved among avian and human influenza viruses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2699363Documentos Relacionados
- The influenza A virus PB2 polymerase subunit is required for the replication of viral RNA.
- The PA Subunit Is Required for Efficient Nuclear Accumulation of the PB1 Subunit of the Influenza A Virus RNA Polymerase Complex
- Mutational analysis identifies functional domains in the influenza A virus PB2 polymerase subunit.
- The RNA polymerase PB2 subunit is not required for replication of the influenza virus genome but is involved in capped mRNA synthesis.
- The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.