Structural organization of upstream exons and distribution of transcription start sites in the chicken c-myb gene.
AUTOR(ES)
Hahn, S L
RESUMO
We mapped and sequenced three upstream exons of the chicken c-myb gene and the regions flanking the first coding exon. We found multiple potential binding sites for transcription factors in the 5'-noncoding region, a T-rich stretch of 78 base pairs (bp) (68% T) in the first intron, and four fairly long open reading frames in the antisense direction of the first coding exon and its flanking regions. Three major transcription start sites, contained within a single 11-bp region, were identified by S1 nuclease analysis and primer extension. A sequence comparison of the avian and murine c-myb genes revealed a highly conserved sequence of 124 bp in the 5'-noncoding region. Its location between the putative transcription factor binding sites and the major transcription start sites suggests that it may play an important regulatory role in c-myb expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=362664Documentos Relacionados
- Positive autoregulation of c-myb expression via Myb binding sites in the 5' flanking region of the human c-myb gene.
- Alternative splicing of RNAs transcribed from the human c-myb gene.
- Extension of the DNA binding consensus of the chicken c-Myb and v-Myb proteins.
- The carboxyterminus of human c-myb protein stimulates activated transcription in trans.
- Human c-myb protooncogene: nucleotide sequence of cDNA and organization of the genomic locus.