Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld.
AUTOR(ES)
Evans, G A
RESUMO
A genomic clone encoding H-2Ld, a mouse major transplantation antigen, has been identified and the structure of the H-2Ld gene has been partially determined. We isolated 35 genomic clones from a BALB/c (H-2d) genomic library by hybridization to mouse or human probes. One of these clones encodes H-2Ld as determined by two criteria. First, the gene encodes a protein that is identical at the 76 known amino acid positions for H-2Ld. Second, when introduced into L cells by DNA-mediated gene transfer, a new H-2 antigen is expressed that is recognized by anti-H-2Ld monoclonal antibodies. The sequence of the H-2Ld protein predicted by the DNA sequences shows more than 80% homology to known H-2 antigens. H-2L-like sequences are found in mutant H-2Kb molecules, suggesting that gene conversion or reciprocal recombination may play a role in the development of H-2 polymorphism.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=346108Documentos Relacionados
- Hormonal regulation of cell surface expression of the major histocompatibility antigen H-2Ld in transfected cells.
- cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.
- A novel downstream regulatory element of the mouse H-2Kb class I major histocompatibility gene.
- Random oligonucleotide mutagenesis: application to a large protein coding sequence of a major histocompatibility complex class I gene, H-2DP.
- Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.